Salt tolerance of segregating progenies of a cross between a domesticated salt\nsensitive tomato cultivar (CA4) and a natural salt-tolerant wild-type tomato\nspecies (LA1606) was characterized. The F1 plants from this cross were selfed\nand 120 F2 segregating progenies from the resulting population along with\nparental CA4 and LA1606 plants were evaluated for salt tolerance. These\nplants were irrigated everyday with 185 mM NaCl for 82 days and quantitative\ntraits were quantified including number of flowers, fruit number, fruit\nweight, fruit length, fruit width, fruit set percentage, and total yield. The two\nparental lines were evaluated for the presence of 27 seven independent RAPD\nmarkers and 7 markers were found to be polymorphic for the two genotypes.\nBulk Segregant (BSA) analyses consisting of pooling 10 â??most tolerantâ? and\n10 â??most sensitiveâ? F2 segregating plants showed association of two RAPD\npolymorphic markers with higher salt tolerance. Two DNA markers that exhibit\nco-segregation with salt tolerance were identified and characterized.\nRAPD marker OPX-17 and MRTOMR-022 exhibited 2 positive molecule\nmarkers (polymorphism) which were found only in the resistant parent\n(LA1606) and resistant F2 bulk.
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